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南京大学唐朝生教授团队:基于电阻率层析成像的微生物诱导碳酸钙沉淀时空分布重构

  • 2026-04-20 14:45:33
南京大学唐朝生教授团队:基于电阻率层析成像的微生物诱导碳酸钙沉淀时空分布重构

标       题:Reconstructing the spatiotemporal distribution of microbial-induced carbonate precipitation using electrical conductivity tomography

期       刊:Engineering Geology

第一作者:Junzheng Zhang(南京大学:地球科学与工程学院)

通讯作者:Chaosheng Tang(南京大学:地球科学与工程学院

出版日期:2026.3.19

2026年影响因子与分区:8.4/Q1

DOI:https://doi.org/10.1016/j.enggeo.2026.108689

摘要

微生物诱导碳酸钙沉淀是一种自然现象,在土木与环境工程等领域具有广泛的应用前景。碳酸钙的时空分布是评价微生物诱导碳酸钙沉淀效果的关键因素。然而,目前尚无合适的无损方法能够实时表征微生物诱导碳酸钙沉淀处理土体中碳酸钙的分布规律,这制约了对微生物诱导碳酸钙沉淀动力学的深入理解。本研究利用电阻率层析成像技术,实现了碳酸钙沉淀时空分布的重构。对实验室尺度的三组硅珠试样开展了多循环微生物诱导碳酸钙沉淀处理,测定了每个处理循环后的流出液电导率及最终碳酸钙体积分数

       结果表明:尿素水解与碳酸钙沉淀对硅珠堆电导率存在相互制约的影响。建立了描述硅珠堆电导率与碳酸钙体积分数关系的岩石物理模型。该模型为微生物诱导碳酸钙沉淀处理土体中碳酸钙分布、孔隙度及胶结指数的实时监测提供了理论依据。基于电阻率层析图像,重构了硅珠堆中碳酸钙的时空分布。研究结果揭示了碳酸钙分布非均匀性的特征及其潜在成因,为深入理解微生物诱导碳酸钙沉淀动力学及促进现场工程管理提供了新途径。

主要图表

Fig. 1. Sketch and photo of the experimental setup. (a) Silica beads were packed into the acrylic box (60 × 10 × 180 mm). (b) Fifty-two stainless electrodes were installed on the backboard of the box, which was used for the electrical conductivity measurement and tomography. (c) Specimens B2 and B3 have the permeability anomaly at the center of the box, which is an acrylic sheet and a polyurethane (PU) foam, respectively. (d)-(g) Photos of the electrical measurement system (ERT21), acrylic box, electrode backboard, and stainless electrodes.

Fig. 2. Procedure of MICP treatment in one cycle (a)-(d) and the correlated electrical monitoring scheme (e). The specimen has a pore volume (PV) of 55 cm3 .

Fig. 3. Determination of the investigated domain.

Fig. 4. Effluent conductivity (cementation solution conductivity) after each MICP cycle in the three specimens (B1, B2, and B3). Since the effluents were recycled for the next MICP cycle (see Fig. 2c), the increase in effluent conductivity can indicate the increase in ion concentration and the progress of mineralization reactions.

Fig. 5. The actual CaCO3 volume fraction after all MICP treatment.

Fig. 6. The evolution of average specimen conductivity with time during each treatment cycle.

Fig. 7. The liquid level in the specimen with injected fluids of different volumes (a) and conductivity tomograms during the cementation solution (CS) injection (b)- (d). The low conductivity zone corresponded to the specimen saturated with bacterial suspension (BS), while the high conductivity zone corresponded to the specimen saturated with CS. The injection of CS started at 160 min with a rate of 0.5 mL/min. 15 mL of CS was injected at 190 min, and 30 mL of CS was injected at 220 min. These two levels of CS are indicated by light-blue solid lines in (c) and (d). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 8. Specimen average conductivity after each MICP treatment cycle.

Fig. 9. Electrical conductivity tomograms before and after each MICP treatment cycle.

Fig. 10. Resampling the interpreted conductivity model before MICP treatment.

Fig. 11. . Cementation exponent m of resampled silica beads pack before and after the MICP treatment (a-b).(c) The cementation exponent m and CaCO3 volume fraction φc of the cemented silica beads were fitted with Eq. 9.

Fig. 12. Petrophysical model between electrical conductivity and CaCO3 volume fraction in MICP-treated silica bead pack.

Fig. 13. Spatiotemporal evolution of the volume fraction of CaCO3 for the three specimens after each MICP treatment cycle.

Fig. 14. Evolution of the average CaCO3 volume fraction φc, porosity ϕ, and cementation exponent m of the silica bead packs after each MICP cycle. φc was estimated using the average electrical conductivity after each MICP cycle based on the proposed petrophysical model (Eq. 10). ϕ and m can be determined based on Eqs. 5 and 9.

研究结论

       本研究采用电阻率层析成像技术,探究了微生物诱导碳酸钙沉淀过程中碳酸钙沉淀的时空分布规律。对实验箱内硅珠堆开展了多循环微生物诱导碳酸钙沉淀处理,在整个处理过程中连续测量电导率。通过流体电导率测量和酸溶法,补充了试样在处理过程中及处理后的电学与物理信息。表征并讨论了微生物诱导碳酸钙沉淀处理过程中试样电导率的动态演化规律。建立了描述电导率与碳酸钙体积分数关系的岩石物理模型,实现了硅珠堆内碳酸钙含量、孔隙度及胶结指数时空分布的重构。主要结论如下:

       1.尿素水解与碳酸钙沉淀对微生物诱导碳酸钙沉淀处理土体电导率存在相互制约的影响。尿素水解通过提高离子浓度主导土体电导率的增加,而碳酸钙沉淀则通过消耗离子并复杂化孔隙结构降低土体电导率。在连续注浆条件下,尿素水解、碳酸钙沉淀及孔隙水驱替的耦合效应导致土体电导率持续降低,表明电导率降低与碳酸钙沉淀之间存在潜在关联。

       2.建立了描述电导率与碳酸钙体积分数关系的岩石物理模型。观察并模拟了胶结指数与碳酸钙体积分数之间的正相关关系,这是电导率-碳酸钙体积分数岩石物理模型的关键组成部分。该岩石物理模型能够估算微生物诱导碳酸钙沉淀处理过程中任意时刻的碳酸钙体积分数。

       3.重构了微生物诱导碳酸钙沉淀处理硅珠堆中碳酸钙沉淀的时空分布。据我们所知,这是首次利用地球物理方法确定碳酸钙沉淀的时空分布。这一进展凸显了地球物理方法在土体固化监测中提供潜在认识的应用价值。

       4.实际与重构碳酸钙分布结果表明,细菌脲酶活性与处理溶液流路是导致微生物诱导碳酸钙沉淀处理土体非均匀性的两个重要原因。脲酶活性影响矿化反应速率及碳酸钙沉淀量。渗流场(包括方向与路径)是导致土体中碳酸钙局部化分布的可能原因之一。

       本研究论证了电阻率层析成像技术在评估微生物诱导碳酸钙沉淀过程与效果方面的应用前景。得益于电阻率层析成像技术表征地下碳酸盐时空分布的能力,可促进微生物诱导碳酸钙沉淀技术的数值模拟应用,特别是砂性土改良(如地基加固与液化 mitigation)。此外,可定性推断处理溶液在低渗透性地层(如粘土与粉土)中的入渗过程。此外,建立岩石物理模型及评估其在微生物诱导碳酸钙沉淀监测中可行性的框架,可拓展至其他土体固化应用,如酶诱导碳酸钙沉淀、水泥固化及植物根系固化。

       为进一步理解微生物诱导碳酸钙沉淀处理土体的非均匀性,仍有若干问题有待解答:(1)如何表征附着于颗粒材料上的细菌分布与活性?(2)孔隙堵塞影响下处理溶液流路的动态演化规律是什么?(3)碳酸钙沉淀的微观结构(如矿物类型与晶体形态)是否影响微生物诱导碳酸钙沉淀处理土体的均匀性?我们认为,电阻率层析成像和频谱激电等地电方法有望在未来解决这些问题。

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  1. CONNECT:[ UseTime:0.000612s ] mysql:host=127.0.0.1;port=3306;dbname=b460;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.000855s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000307s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000291s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.000456s ]
  6. SELECT * FROM `set` [ RunTime:0.000206s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.000518s ]
  8. SELECT * FROM `article` WHERE `id` = 534289 LIMIT 1 [ RunTime:0.000906s ]
  9. UPDATE `article` SET `lasttime` = 1776761688 WHERE `id` = 534289 [ RunTime:0.001268s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000219s ]
  11. SELECT * FROM `article` WHERE `id` < 534289 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000429s ]
  12. SELECT * FROM `article` WHERE `id` > 534289 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.000379s ]
  13. SELECT * FROM `article` WHERE `id` < 534289 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000705s ]
  14. SELECT * FROM `article` WHERE `id` < 534289 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.000708s ]
  15. SELECT * FROM `article` WHERE `id` < 534289 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.000665s ]
0.076882s