当前位置:首页>南京>PBJ | 南京农大王源超教授/华中农大朱龙付教授合作发现免疫识别受体RXEG1通过保守的识别机制赋予作物广谱抗病性

PBJ | 南京农大王源超教授/华中农大朱龙付教授合作发现免疫识别受体RXEG1通过保守的识别机制赋予作物广谱抗病性

  • 2026-05-29 13:49:23
PBJ | 南京农大王源超教授/华中农大朱龙付教授合作发现免疫识别受体RXEG1通过保守的识别机制赋予作物广谱抗病性
病原菌引起的植物病害对粮食生产安全构成重大威胁。系统解析植物-病原菌互作的分子机制,深入挖掘植物抗病基因资源并定向改良作物抗性是实现病害有效防控的重要手段。植物进化出一套先天免疫系统用于监测和抵御病原菌的入侵。植物细胞膜表面的模式识别受体(Pattern recognition receptors,PRRs)能够识别包括病原/微生物相关分子模式(Pathogen/Microbe-associated molecular pattern,PAMPs/MAMPs)或损伤相关分子模式(Damage associated molecular patterns,DAMPs)等危险信号,迅速激活病原菌相关分子模式触发的免疫反应(Pattern-triggered immunity,PTI),为植物构筑起第一道防线。
南京农业大学王源超教授团队在前期的研究中发现疫霉菌在侵染植物早期分泌的核心效应分子糖苷水解酶家族12蛋白(Glycoside hydrolase 12,GH12)XEG1通过降解细胞壁破坏植物抗病性(Ma et al., 2015)。进一步研究发现,在进化过程中,疫霉菌获得了XEG1的酶活丧失突变体XLP1,利用XLP1作为“分子诱饵”竞争性保护核心效应分子XEG1免受植物蛋白酶抑制子GIP1的攻击,揭示了病原菌致病新机制“诱饵模式”(Ma et al., 2017)。随后的研究发现大豆疫霉通过N-糖基化修饰来保护XEG1免受植物胞外天冬氨酸蛋白酶GmAP5的降解(Xia et al., 2020)。XEG1可被烟草、大豆、番茄等多种植物识别诱导免疫反应(Ma et al., 2015)。为了解析XEG1的免疫识别机制,该团队成功鉴定到XEG1的免疫识别受体RXEG1(Wang et al., 2018)。通过解析识别受体RXEG1多种不同状态的结构并结合体内生化和功能分析,揭示了XEG1通过别构效应促进RXEG1与受体蛋白激酶BAK1异源二聚化激活植物抗性的作用机制(Sun et al., 2022)。GH12蛋白在病原微生物中广泛存在(Ma et al., 2015),RXEG1能否识别不同病原菌GH12蛋白发挥广谱抗性尚不清楚。近日,王源超教授团队联合朱龙付教授团队在国际权威学术期刊《Plant Biotechnology Journal》发表题为《The receptor-like protein RXEG1 confers broad-spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins》的研究论文,系统解析了RXEG1识别不同病原菌GH12蛋白保守的分子机制,通过将RXEG1导入大豆及棉花,证实了RXEG1利用其对不同病原菌GH12蛋白保守的“免疫识别”和“酶活抑制”功能增强不同作物对多种重要病原菌的抗性。
该研究发现RXEG1可识别来自多种病原真菌和卵菌的GH12蛋白。结构预测与关键互作位点突变分析表明,尽管这些GH12蛋白在序列上存在较大变异,RXEG1仍能通过其N-loopout以及C端的岛区(ID-domain)结构域,与GH12蛋白保守的底物识别位点和酶活性位点相互作用,形成保守的互作界面。此外,RXEG1通过结合在GH12蛋白酶活口袋中,广泛抑制不同GH12蛋白的木葡聚糖水解酶活性。利用农杆菌介导的稳定转化体系,该研究构建了RXEG1转基因大豆和棉花株系。接种实验发现过表达RXEG1能有效提高大豆对大豆疫霉、豆薯层锈菌、拟茎点种腐病菌、镰刀菌等多种病原真菌与卵菌的抗性,以及棉花对大丽轮枝菌的抗性。并且,过表达RXEG1没有显著影响大豆和棉花的株高、单株产量等农艺性状。以上研究表明,模式识别受体基因RXEG1在大豆和棉花等重要作物的遗传改良方面具有重要的应用潜力。

1. RXEG1结合GH12蛋白酶活口袋并抑制其木葡聚糖水解酶活性

2. 过表达RXEG1提高大豆和棉花对不同病原菌的抗性

南京农业大学曾梦竹博士和湖南农业大学邱萍博士为论文的共同第一作者,王源超教授、朱龙付教授、王燕教授和段凯旋副教授为本文的共同通讯作者,安徽农科院赵伟研究员也参与了此项研究。本研究得到了国家自然科学基金、国家重点研发计划、钟山生物育种实验室项目的资助。

本文链接:

https://doi.org/10.1111/pbi.70139

参考文献:

1、 Ma Z, Song T, Zhu L, Ye W, Wang Y, Shao Y, Dong S, Zhang Z, Dou D, Zheng X, Tyler BM, and Wang YC*. (2015) A Phytophthora sojae glycoside hydrolase 12 protein is a major virulence factor during soybean infection and is recognized as a PAMP. Plant Cell 27:2057-2072.

2、 Ma Z, Zhu L, Song T, Wang Y, Zhang Q, Xia Y, Qiu M, Lin Y, Li H, Kong L, Fang Y, Ye W, Dong S, Zheng X, Tyler BM, and Wang YC*. (2017) A paralogous decoy protects Phytophthora sojae apoplastic effector PsXEG1 from a host inhibitor. Science 355:710-714.

3、 Xia Y, Ma Z, Qiu M, Guo B, Zhang Q, Jiang H, Zhang B, Lin Y, Xuan M, Sun L, Shu H, Xiao J, Ye W, Wang Y, Wang Y, Dong S, Tyler BM, and Wang YC*. (2020) N-glycosylation shields Phytophthora sojae apoplastic effector PsXEG1 from a specific host aspartic protease. PNAS 117, 27685-27693.

4、 Wang Y, Xu Y, Sun Y, Wang H, Qi J, Wan B, Ye W, Lin Y, Shao Y, Dong S, Tyler BM, and Wang YC*. (2018) Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection. Nature Communications 9:594.

5、 Sun Y, Wang Y*, Zhang X, Chen Z, Xia Y, Wang L, Sun Y, Zhang M, Xiao Y, Han Z*, Wang YC*, and Chai J*. (2022) Plant receptor-like protein activation by a microbial glycoside hydrolase. Nature 610(7931):335-342.

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  1. CONNECT:[ UseTime:0.000633s ] mysql:host=127.0.0.1;port=3306;dbname=b460;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.001004s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000341s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000300s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.000674s ]
  6. SELECT * FROM `set` [ RunTime:0.000246s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.000782s ]
  8. SELECT * FROM `article` WHERE `id` = 569284 LIMIT 1 [ RunTime:0.000500s ]
  9. UPDATE `article` SET `lasttime` = 1780188323 WHERE `id` = 569284 [ RunTime:0.004850s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000293s ]
  11. SELECT * FROM `article` WHERE `id` < 569284 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000494s ]
  12. SELECT * FROM `article` WHERE `id` > 569284 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.000383s ]
  13. SELECT * FROM `article` WHERE `id` < 569284 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000924s ]
  14. SELECT * FROM `article` WHERE `id` < 569284 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.000696s ]
  15. SELECT * FROM `article` WHERE `id` < 569284 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.000748s ]
0.084882s